Simply switch to Pierce ECL and save a bundle.ĮCL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probesĪbout half the cost of other ECL Substrates – low overhead and a commitment to customer value enables Thermo Fisher Scientific to make this product available to you at a significantly lower price than other companies (these claims are based on the 2010 U.S. Western blotting is a powerful technique that enables indirect detection of protein samples immobilized on a nitrocellulose or polyvinylidene fluoride (PVDF) membranes. The FastGene Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol. To make a working solution, the two components are mixed together. Because the luminol and peroxide reagent-formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Enhanced chemiluminescence (ECL) substrates, are HRP substrates that are typically two-component systems consisting of a stable peroxide solution and an enhanced luminol solution. Most other pico level ECL solution has high back ground. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. Provides maximum sensitivity in blotting application by allowing detection in the pico-gram range. Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard enhanced chemilumescent (ECL) substrates for detection of horseradish peroxidase (HRP) enzyme activity, yet costs much less. Western blot is often used in research to separate and identify proteins. 1 ml Tris, 9 ml steril UP water in sterile tube, mix, add 22.5 microL p-coumaric acid, mix, then add 50 microL luminol, mix, then add. Incubate membrane with ECL solution (GE Healthcare, Piscataway, NJ. Thermo Scientific™ Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. I always make the reagent from stock promptly before detection.
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